%var: 'egf_tot' 1.2e6 # molecule counts %var: 'egfr_tot' 1.8e5 # molecule counts %var: 'Grb2_tot' 1.0e5 # molecule counts %var: 'Shc_tot' 2.7e5 # molecule counts %var: 'Sos_tot' 1.3e4 # molecule counts %var: 'Grb2_Sos_tot' 4.9e4 # molecule counts %var: 'kp1' 1.667e-06 # ligand-monomer binding (scaled), units: /molecule/s %var: 'km1' 0.06 # ligand-monomer dissociation, units: /s %var: 'kp2' 5.556e-06 # aggregation of bound monomers (scaled), units: /molecule/s %var: 'km2' 0.1 # dissociation of bound monomers, units: /s %var: 'kp3' 0.5 # dimer transphosphorylation, units: /s %var: 'km3' 4.505 # dimer dephosphorylation, units: /s %var: 'kp14' 3 # Shc transphosphorylation, units: /s %var: 'km14' 0.03 # Shc dephosphorylation, units: /s %var: 'km16' 0.005 # Shc cytosolic dephosphorylation, units: /s %var: 'kp9' 8.333e-07 # binding of Grb2 to receptor (scaled), units: /molecule/s %var: 'km9' 0.05 # dissociation of Grb2 from receptor, units: /s %var: 'kp10' 5.556e-06 # binding of Sos to receptor (scaled), units: /molecule/s %var: 'km10' 0.06 # dissociation of Sos from receptor, units: /s %var: 'kp11' 1.25e-06 # binding of Grb2-Sos to receptor (scaled), units: /molecule/s %var: 'km11' 0.03 # diss. of Grb2-Sos from receptor, units: /s %var: 'kp13' 2.5e-05 # binding of Shc to receptor (scaled), units: /molecule/s %var: 'km13' 0.6 # diss. of Shc from receptor, units: /s %var: 'kp15' 2.5e-07 # binding of ShcP to receptor (scaled), units: /molecule/s %var: 'km15' 0.3 # diss. of ShcP from receptor, units: /s %var: 'kp17' 1.667e-06 # binding of Grb2 to RP-ShcP (scaled), units: /molecule/s %var: 'km17' 0.1 # diss. of Grb2 from RP-ShcP, units: /s %var: 'kp18' 2.5e-07 # binding of ShcP-Grb2 to receptor (scaled), units: /molecule/s %var: 'km18' 0.3 # diss. of ShcP-Grb2 from receptor, units: /s %var: 'kp19' 5.556e-06 # binding of Sos to RP-ShcP-Grb2 (scaled), units: /molecule/s %var: 'km19' 0.0214 # diss. of Sos from RP-ShcP-Grb2, units: /s %var: 'kp20' 6.667e-08 # binding of ShcP-Grb2-Sos to receptor (scaled), units: /molecule/s %var: 'km20' 0.12 # diss. of ShcP-Grb2-Sos from receptor, units: /s %var: 'kp24' 5e-06 # binding of Grb2-Sos to RP-ShcP (scaled), units: /molecule/s %var: 'km24' 0.0429 # diss. of Grb2-Sos from RP-ShcP, units: /s %var: 'kp21' 1.667e-06 # binding of ShcP to Grb2 in cytosol (scaled), units: /molecule/s %var: 'km21' 0.01 # diss. of Grb2 and SchP in cytosol, units: /s %var: 'kp23' 1.167e-05 # binding of ShcP to Grb2-Sos in cytosol (scaled), units: /molecule/s %var: 'km23' 0.1 # diss. of Grb2-Sos and SchP in cytosol, units: /s %var: 'kp12' 5.556e-08 # binding of Grb2 to Sos in cytosol (scaled), units: /molecule/s %var: 'km12' 0.0015 # diss. of Grb2 and Sos in cytosol, units: /s %var: 'kp22' 1.667e-05 # binding of ShcP-Grb2 to Sos in cytosol (scaled), units: /molecule/s %var: 'km22' 0.064 # diss. of ShcP-Grb2 and Sos in cytosol, units: /s %init: 'egf_tot' egf(r) %init: 'egfr_tot' egfr_1(l,r) %init: 'egfr_tot' egfr_2(l,r,Y1068~Y) %init: 'egfr_tot' egfr_3(l,r,Y1148~Y) %init: 'Grb2_tot' Grb2(SH2,SH3) %init: 'Shc_tot' Shc(PTB,Y317~Y) %init: 'Sos_tot' Sos(dom) # Ligand-receptor binding egfr_1(l,r) , egf(r) <-> egfr_1(l!1,r),egf(r!1) @'kp1'{0}, 'km1' #ligand-monomer egfr_2(l,r) <-> egfr_2(l!1,r),egf(r!1) @'kp1'*|egf(r)|, 'km1' #ligand-monomer egfr_3(l,r) <-> egfr_3(l!1,r),egf(r!1) @'kp1'*|egf(r)|, 'km1' #ligand-monomer # Receptor-aggregation egfr_2(l!_,r) , egfr_1(l!_,r) <-> egfr_2(l!_,r!3),egfr_1(l!_,r!3) @'kp2'{0}, 'km2' egfr_3(l!_,r) <-> egfr_3(l!_,r!3),egfr_1(l!1,r!3),egf(r!1) @'kp2'*|egf(r!1),egfr_1(l!1)|, 'km2' # Transphosphorylation of egfr by RTK egfr_2(r!_,Y1068~Y) -> egfr_2(r!_,Y1068~pY) @'kp3' egfr_3(r!_,Y1148~Y) -> egfr_3(r!_,Y1148~pY) @'kp3' # Dephosphorylation egfr_2(Y1068~pY) -> egfr_2(Y1068~Y) @'km3' egfr_3(Y1148~pY) -> egfr_3(Y1148~Y) @'km3' # Shc transphosph egfr_3(r!_,Y1148~pY!1),Shc(PTB!1,Y317~Y) -> egfr_3(r!_,Y1148~pY!1),Shc(PTB!1,Y317~pY) @'kp14' Shc(PTB!_,Y317~pY) -> Shc(PTB!_,Y317~Y) @'km14' # Y1068 activity egfr_2(Y1068~pY) , Grb2(SH2,SH3) <-> egfr_2(Y1068~pY!1),Grb2(SH2!1,SH3) @'kp9'{0}, 'km9' egfr_2(Y1068~pY) , Grb2(SH2,SH3!_) <-> egfr_2(Y1068~pY!1),Grb2(SH2!1,SH3!_) @'kp11'{0}, 'km11' egfr_2(Y1068~pY!1),Grb2(SH2!1,SH3) , Sos(dom) <-> egfr_2(Y1068~pY!1),Grb2(SH2!1,SH3!2),Sos(dom!2) @'kp10', 'km10' # Y1148 activity egfr_3(Y1148~pY) , Shc(PTB,Y317~Y) <-> egfr_3(Y1148~pY!1),Shc(PTB!1,Y317~Y) @'kp13'{0}, 'km13' egfr_3(Y1148~pY) , Shc(PTB,Y317~pY) <-> egfr_3(Y1148~pY!1),Shc(PTB!1,Y317~pY) @'kp15'{0}, 'km15' egfr_3(Y1148~pY) , Shc(PTB,Y317~pY!1),Grb2(SH2!1,SH3) <-> \ egfr_3(Y1148~pY!2),Shc(PTB!2,Y317~pY!1),Grb2(SH2!1,SH3) @'kp18'{0}, 'km18' egfr_3(Y1148~pY) , Shc(PTB,Y317~pY!1),Grb2(SH2!1,SH3!3),Sos(dom!3) <-> \ egfr_3(Y1148~pY!2),Shc(PTB!2,Y317~pY!1),Grb2(SH2!1,SH3!3),Sos(dom!3) @'kp20'{0}, 'km20' egfr_3(Y1148~pY!1),Shc(PTB!1,Y317~pY) , Grb2(SH2,SH3) <-> \ egfr_3(Y1148~pY!1),Shc(PTB!1,Y317~pY!2),Grb2(SH2!2,SH3) @'kp17'{0}, 'km17' egfr_3(Y1148~pY!1),Shc(PTB!1,Y317~pY) , Grb2(SH2,SH3!3),Sos(dom!3) <-> \ egfr_3(Y1148~pY!1),Shc(PTB!1,Y317~pY!2),Grb2(SH2!2,SH3!3),Sos(dom!3) @'kp24', 'km24' Shc(PTB!_,Y317~pY!2),Grb2(SH2!2,SH3) , Sos(dom) <-> \ Shc(PTB!_,Y317~pY!2),Grb2(SH2!2,SH3!3),Sos(dom!3) @'kp19'{0}, 'km19' # Cytosolic Shc(PTB,Y317~pY) , Grb2(SH2,SH3) <-> Shc(PTB,Y317~pY!1),Grb2(SH2!1,SH3) @'kp21'{0}, 'km21' Shc(PTB,Y317~pY) , Grb2(SH2,SH3!_) <-> Shc(PTB,Y317~pY!1),Grb2(SH2!1,SH3!_) @'kp23'{0}, 'km23' Shc(PTB,Y317~pY) -> Shc(PTB,Y317~Y) @'km16' Grb2(SH2,SH3) , Sos(dom) <-> Grb2(SH2,SH3!1),Sos(dom!1) @'kp12'{0}, 'km12' Shc(PTB,Y317~pY!2),Grb2(SH2!2,SH3) , Sos(dom) <-> \ Shc(PTB,Y317~pY!2),Grb2(SH2!2,SH3!3),Sos(dom!3) @'kp22', 'km22'